Transfer of Fv-1 locus-specific resistance to murine N-tropic and B-tropic retroviruses by cytoplasmic RNA
نویسندگان
چکیده
منابع مشابه
Reversal of Fv-1 host range by in vitro restriction endonuclease fragment exchange between molecular clones of N-tropic and B-tropic murine leukemia virus genomes.
We molecularly cloned unintegrated viral DNA of the BALB/c endogenous N-tropic and B-tropic murine leukemia retroviruses and in vitro passaged N-tropic Gross (passage A) murine leukemia retroviruses. Recombinant genomes were constructed in vitro by exchanging homologous restriction enzyme fragments from N- or B-tropic parents and subsequent recloning. Infectious virus was recovered after transf...
متن کاملLate-passage SC-1 cells resistant to N- and B-tropic viruses but sensitive to NB-tropic viruses.
A late-passage derivative of SC-1 cells with stable relative resistance to N- and B-tropic viruses but with retained sensitivity to NB-tropic viruses has been identified. Dual resistance was seen with the XC plaque assay, as well as reverse transcriptase assay and radioimmunoassay for viral p30. Evidence was found for resistance in clonal derivatives. These findings suggest a possible instabili...
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Production of various forms of nonintegrated viral DNA was measured in cultured mouse cells carrying different Fv-1 alleles early after infection with N-tropic or B-tropic retroviruses. Quantitative analyses were performed by agarose gel electrophoresis, transfer to diazobenzyloxymethyl-paper, and molecular hybridization. In permissive infection of Fv-1n cells (NIH Swiss and DBA mouse strains) ...
متن کاملNew gene locus modifying susceptibility to certain B-tropic murine leukemia viruses.
A new gene, SRV, the dominant allele of which occurs in mice of strain C57BL, independently modifies the slope ("hitness") and level of sensitivity of the titration curves obtained when one subclass of B-tropic murine leukemia viruses is propagated on mouse embryo cells of nonpermissive Fv-1 genotypes. Replication of another subclass of B-tropic viruses is not modified with respect to hitness, ...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1978
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.27.2.288-299.1978